Place the membrane between two pieces of dry filter paper, and place the membrane and filter papers into a protected place overnight.Leave the membrane and filter paper in a 37 ☌ oven for about 10 minutes. Place the membrane on a piece of dry filter paper.Leave the membrane and filter paper on the benchtop for about an hour. Blots can be dried using any of the following options. Dry MembraneĪllow blots to dry before proceeding with detection. More information about transferring proteins to a membrane is available here /proteintransfer. Transfer to nitrocellulose or PVDF membrane. Run the gel according to the manufacturer's instructions. For example, you may need to use a different starting value for your dilution series to ensure that your target can be detected in every dilution. The actual amounts you load may vary depending on the particular conditions in your experiment. Using two 15-well gels, load the following samples in the order indicated in the table below. You will be loading a serial dilution in duplicate and in random sample order. Use only a pencil to write on PVDF membranes, because the ink from the Odyssey Pen will dissolve in the methanol used to wet the PVDF membrane.įollowing is a suggested template for loading a gel for the blocker optimization experiment. You can write on nitrocellulose membranes with pencil or the Odyssey Pen (PN 926-71804).
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